イベント 2019.10.21


日時:2019年10月31日(木) 16:30 — 18:00

グリーン科学技術研究所 成川研究室主催 講演会を開催します

【日 時】

【場 所】
静岡大学静岡キャンパス 理学部A棟2階 大会議室

【講 師】
Matthias Rögner教授

Remodeling of photosynthetic electron transport in Synechocystis PCC 6803 for future hydrogen production from water

【概 要】
Photosynthetic microorganisms such as the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) can be exploited for the light-driven synthesis of valuable compounds. Energetically, this is much more rewarding if photosynthetic electrons are branched-off at Ferredoxin (Fd), which provides electrons for a variety of fundamental metabolic pathways in the cell, with the Ferredoxin-NADP-Oxido-Reductase (FNR, PetH) being the main target. In order to re-direct electrons from Fd to another consumer, the high electron transport rate between Fd and FNR has to be weakened [1]. Based on our previous in vitro experiments, corresponding FNR-mutants at position FNR_K190 [2] have now been generated in Synechocystis cells to study their impact on the cellular metabolism and their potential for a future hydrogen producing design cell. Out of two promising candidates, mutation FNR_K190D proved to be lethal due to oxidative stress, while FNR_K190A was successfully generated and characterized: The light induced NADPH formation is clearly impaired in this mutant and it shows also major metabolic adaptations like a higher glucose metabolism as evidenced by quantitative mass spectrometric analysis. These results indicate a high potential for the future use of photosynthetic electrons in engineered design cells – for instance for hydrogen production. They also reveal substantial differences in the interaction of proteins if characterized in an in vitro environment [3] in comparison with the physiological conditions of whole cells which have to be considered in remodeling processes.

1. Rögner M (2013) Biochem Soc Trans, 2013. 41(5), 1254-9
2. Wiegand K et al. (2018) Biochim Biophys Acta 1859(4), 253-262
3. Kothe T et al. (2013) Angew Chem Int Ed 52, 14233-14236

静岡大学 学術院 理学専攻 光生物学研究室(成川研究室)